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ENRICHMENT AND ISOLATION OF MICROBIAL CELLS AND MICROBIAL NUCLEIC ACIDS FROM A BIOLOGICAL SAMPLE
ENRICHMENT AND ISOLATION OF MICROBIAL CELLS AND MICROBIAL NUCLEIC ACIDS FROM A BIOLOGICAL SAMPLE
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机译:从生物样品中富集和分离微生物细胞和微生物核酸
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摘要
A method for enrichment and isolation of microbial cells and microbial nucleic acids from a biological sample is described. The method comprises (i) adding to an initial volume of biological sample a differential cell lysis solution to obtain a final concentration of 0.1 to 1% of SDS in the sample; (ii) mixing the solution obtained in step (i) for a period of time sufficient to lyse the host cells present in the biological sample, while preserving the integrity of cells; and (iii) separating the microbial cells from the lysed host cells components. Differential cell lysis solutions and kits for practicing the method of the present invention are also provided.
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