METHODS FOR INCREASING THE FREQUENCY OF GENE TARGETING BY CHROMATIN MODIFICATION

摘要

The present disclosure relates to methods and kits for increasing the frequency of insertion of a nucleotide sequence of interest in a specific chromosomal site within the genome of a eukaryotic cell by modifying the state of chromatin in said cell. In the present disclosure chromatin state may be altered by reducing nucleosomal occupancy by modulating the activity of high-mobility group box (HMGB) proteins.