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LEADER-MODIFIED GLUCOAMYLASE POLYPEPTIDES AND ENGINEERED YEAST STRAINS HAVING ENHANCED BIOPRODUCT PRODUCTION
LEADER-MODIFIED GLUCOAMYLASE POLYPEPTIDES AND ENGINEERED YEAST STRAINS HAVING ENHANCED BIOPRODUCT PRODUCTION
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机译：领先的改性葡糖淀粉酶多肽和工程化酵母菌，可提高生物制品的产量
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摘要
The invention is directed to non-natural yeast able to secrete significant amounts of glucoamylase into a fermentation media. The glucoamylase can promote degradation of starch material generating glucose for fermentation to a desired bioproduct, such as ethanol. The glucoamylase can be provided in the form of a glucoamylase fusion protein having secretion signal that is: derived from at least AA 1-19 of SEQ ID NO: 73, (ii) an amino acid sequence of at least AA 1-19 of SEQ ID NO: 74, (iii) SEQ ID NO: 77 (An C aa), (iv) SEQ ID NO: 75 (Sc IV), (v) SEQ ID NO: 76 (Gg LZ), or (vi) SEQ ID NO: 78(Hs SA).
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机译：本发明涉及能够将大量的葡糖淀粉酶分泌到发酵培养基中的非天然酵母。葡糖淀粉酶可以促进淀粉物质的降解，产生用于发酵的葡萄糖，以发酵成所需的生物产物，例如乙醇。葡糖淀粉酶可以以具有分泌信号的葡糖淀粉酶融合蛋白的形式提供，该分泌信号是：衍生自SEQ ID NO：73的至少AA 1-19，（ii）SEQ ID NO：73的至少AA 1-19的氨基酸序列ID NO：74，（iii）SEQ ID NO：77（An Caa），（iv）SEQ ID NO：75（Sc IV），（v）SEQ ID NO：76（Gg LZ）或（vi）SEQ编号：78（Hs SA）。
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