RECOVERIN AS A FUSION PROTEIN TAG TO IMPROVE EXPRESSION, SOLUBILITY AND PURIFICATION OF PROTEINS

摘要

Herein presented is a new, versatile fusion protein tool (tag) to improve the solubility and purification of proteins. Particularly, the fusion tag is the substantially full-length (about 23 kD) Recoverin molecule (TagR) that is used for protein purification in a single step, even in the presence of detergents.