PRIMER FOR NEXT GENERATION SEQUENCER AND METHOD FOR PRODUCING THE SAME, DNA LIBRARY OBTAINED THROUGH USE OF PRIMER FOR NEXT GENERATION SEQUENCER AND METHOD FOR PRODUCING THE SAME, AND GENOME DNA ANALYZING METHOD USING DNA LIBRARY

摘要

To provide a primer used for a next-generation sequencer that can provide a large number of reads.SOLUTION: On the basis of the sequence: 5'-CAAGCAGAAGACGGCATACGAGAT-N-GTCTCGTGGGCTCGGAGATGTGTATAAGAGACAG-3', wherein Nindicates an index sequence of 5 to 15 bases, an index sequence is designed as a base sequence exhibiting the putative number of reads, which is calculated using the estimation formula designating the number of reads as a purpose variable and the type of bases in the index sequence as an explanatory variable, exceeding a given level.SELECTED DRAWING: Figure 1