A PRIMER FOR NEXT GENERATION SEQUENCER AND A METHOD FOR PRODUCING THE SAME, A DNA LIBRARY OBTAINED THROUGH THE USE OF A PRIMER FOR NEXT GENERATION SEQUENCER AND A METHOD FOR PRODUCING THE SAME, AND A DNA ANALYZING METHOD USING A DNA LIBRARY

摘要

This invention provides a primer used for a next-generation sequencer that can provide a large number of reads.　On the basis of the sequence: 5'-CAAGCAGAAGACGGCATACGAGAT-N_{5 to 15}-GTCTCGTGGGCTCGGAGATGTGTATAAGAGACAG-3', wherein N_{5 to 15} indicates an index sequence of 5 to 15 nucleotides), an index sequence is designed as a nucleotide sequence exhibiting the putative number of reads, which is calculated using the estimation formula designating the number of reads as a purpose variable and the type of nucleotides in the index sequence as an explanatory variable, exceeding a given level.