Evaluation method of skin sensitization in plant extracts

摘要

To provide a method for evaluating skin sensitization to plant extract that has not been detected so far in an in-vitro evaluation system of a sensitizing substance using a cell.SOLUTION: The in-vitro evaluation method or screening method of a sensitizing substance, adding and exposing a test object that is supposed to include a sensitizing substance in a cell to detect a molecule expressed on a surface of he cell, is characterized in that the test object that is supposed to include the sensitizing substance is a plant extract, a concentration of the plant extract to be added is 5000 μg/ml-500000 μg/ml, and an exposure time is 1-30 minutes. The in-vitro evaluation method includes a process of a skin sensitization evaluation method to the plant extract without using an animal, which has been hard to evaluate so far.SELECTED DRAWING: Figure 2