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Bacterial community relationship determination and other high-throughput microbiology applied high resolution systems, kits, devices, and methods
Bacterial community relationship determination and other high-throughput microbiology applied high resolution systems, kits, devices, and methods
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机译:细菌群落关系测定和其他高通量微生物学应用高分辨率系统,试剂盒,设备和方法
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摘要
A method is provided for analyzing a sample comprising a population of biological entities using at least one microfabricated device. Each of the plurality of microwells on the microfabricated device is uniquely indexed and the sample is filled such that at least some of the microwells each contain two or more cells of the biological entity. The microfabricated device is incubated under predetermined conditions, and the selected genetic material of the biological entity cells obtained from the incubation is amplified to obtain an amplicon. Sequence amplicon collections, obtain sequencing data, and based on that and based on microwell indexing, obtain identification of biological entities present in each of the plurality of microwells . Such identification may be used to determine relationships between different types of biological entities in the sample.
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