DETECTION METHOD OF CROSS-CONTAMINATION BETWEEN SAMPLES IN NEXT-GENERATION SEQUENCING

摘要

To provide a technology for next-generation sequencing, in which a nucleic acid sample may be determined or identified, and cross-contamination etc. between samples may be quantitatively confirmed.SOLUTION: There is provided a method for preparing a library containing nucleic acid molecule fragments derived from a plurality of subjects, which is subjected to a nucleobase sequencing method where a plurality of sequencing reactions are performed in parallel. The method comprises: a step where each of nucleic molecules derived from respective of subjects is mixed with spike-in DNA specific to the subjects so as to prepare a library nucleic acid sample associated with each of the subjects; and a step where each of nucleic acid molecules or spike-in DNA prepared from nucleic acid molecules contained in the library nucleic acid sample associated with each of the subjects is provided with barcode sequence consisting of a nucleobase sequence specific to each sample so as to prepare a library derived from each subject. The spike-in DNA comprises an index sequence and an index search sequence consisting of a nucleobase sequence specific to each of the subjects.SELECTED DRAWING: Figure 1