Methods for in vivo high-throughput evaluation of RNA-inducible nuclease activity

摘要

The present invention relates to a method for assessing the activity of RNA-inducible nucleases in cells in a high throughput manner, in particular a cell library comprising a nucleotide sequence encoding a guide RNA and an isolated oligonucleotide comprising the target nucleotide sequence. The present invention relates to a method for evaluating the activity of RNA-inducible nuclease from the indel frequency of Methods for characterizing RNA-inducible nucleases using the guide RNA-target sequence pair library of the invention allow to assess the activity of RNA-inducible nucleases in vivo in a high-throughput manner, thus The method is very effective in all fields where RNA-inducible nucleases are applied.