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Novel microbial herbicidal composition that inhibits water hyacinth reproduction

机译:抑制水葫芦繁殖的新型微生物除草剂

摘要

The present invention deals with a mycoherbicidal isolate of Setosphaeria monoceras sp. or extracts obtained therefrom, useful for the control of Water hyacinth. The present invention also discloses biological control compositions comprising fungal isolate extract formulated in a growth medium for maintaining the viability of extract when the biological control composition is applied to weed. The present invention also discloses methods of screening fungal isolate to determine if they exhibit biocontrol activity. The present invention also discloses use of Setosphaeria monoceras strain. In yet a further aspect the invention relates to use of an Setosphaeria strain for producing a herbicidal agent effective for controlling growth of water hyacinth plants. The strain used preferably is a strain having the characterizing features of Setosphaeria strain AGWH#1 1 as deposited at IMTECH under accession number MTCC 5974. The present invention to provide the 18s rRNA sequence analysis showed 96% nucleotide similarity of the Fungi to Setosphaeria monoceras. (AGBIO designated AGWH#1 1/NCIM1370/MTCC 5974) but also indicated nucleotide variation of this fungus from other known genus of Bipolaris, Pleosporaceae, Cochiliobolus, Alternaria including an environmentally acceptable alternative to synthetic chemical herbicides for the control of weeds, such as Water hyacinth. The present invention also discloses methods of whole genome study of strain.
机译:本发明涉及单孢Seterphaeria monoceras sp。的杀真菌性分离物。或由此获得的提取物,可用于控制风信子。本发明还公开了生物防治组合物,其包含配制在生长培养基中的真菌分离物提取物,以在将生物防治组合物施用于杂草时保持提取物的活力。本发明还公开了筛选真菌分离物以确定它们是否表现出生物防治活性的方法。本发明还公开了单峰Setosphaeria monoceras菌株的用途。在另一方面,本发明涉及门孢菌属菌株在生产有效控制水葫芦植物生长的除草剂中的用途。优选使用的菌株是具有以注册号MTCC 5974保藏在IMTECH上的门孢菌属菌株AGWH#11 1的表征特征的菌株。提供18s rRNA序列分析的本发明显示了真菌与单门孢菌的96%核苷酸相似性。 (AGBIO命名为AGWH#1/1 / NCIM1370 / MTCC 5974),但也指出了该真菌与其他已知的双极类,伞形科,钩藤属,链格孢属的真菌的核苷酸变异,包括一种环境可接受的合成化学除草剂替代品,用于控制杂草,例如水葫芦。本发明还公开了菌株的全基因组研究方法。

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