P27 tyrosine phosphorylation as a marker of CDK4 activity and method for using it

摘要

Compositions and methods for the treatment of malignancies are disclosed. In particular, the disclosure is a method for treating cancer, comprising assessing tyrosine 88 (Y 88) phosphorylation (pY 88) levels of p27 in a biological sample comprising cancer cells from the subject. And stratifying the pY88 phosphorylation level as 0, 1, 2, or 3 as compared to the pY88 phosphorylation level observed in and control tissues, a level of 0 being cyclin dependent kinase 4 (cd 4 k) It shows no detectable sensitivity to inhibition, a level of 1 shows low or no detectable sensitivity, and a level of 2 or 3 provides a way to show detectable sensitivity to cdk4 inhibition. Further provided is a kit for performing the method.