Compositions and methods for diagnosing Lyme disease and for predicting Lyme disease spirochete removal after treatment

摘要

Contacting a synthetic peptide comprising a T cell epitope-containing region derived from a Borrelia protein expressed at different stages of Lyme disease in vitro with a whole blood sample from a subject suspected of having LD and responding to stimulation by the peptide Detecting, diagnosing and prognosing Lyme disease (LD), including indirectly detecting LD-specific activated T cells by determining a T cell immune response indicator (eg, interferon-γ) produced Compositions and methods for prediction are provided. Also, whole blood samples from LD patients undergoing LD treatment are exposed to peptides containing specific T cell epitope regions of Borrelia protein expressed at different stages of Lyme disease, and T cell immune response indicators (eg, Also disclosed is a method for predicting LD spirochete removal in LD patients undergoing LD therapy, confirming that the sample does not contain Borrelia-specific activated T cells because of the absence of interferon-γ). .