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Method of reducing measurement error caused by catalase inhibition by azide

机译:减少由叠氮化物抑制过氧化氢酶引起的测量误差的方法

摘要

Disclosed is a method for reducing measurement errors due to inhibition of catalase by azide in a method for quantification of a component to be measured, in which hydrogen peroxide derived from a component other than the component to be measured is decomposed by a catalase. The method for reducing measurement errors due to inhibition of catalase by azide employs a catalase which has a subunit having a molecular mass of 75 kDa or higher and is derived from a microorganism, when hydrogen peroxide derived from a component other than the component to be measured is decomposed by the catalase followed by quantification of hydrogen peroxide derived from the component to be measured to quantify the component to be measured.
机译:公开了一种在定量被测成分的方法中用于减少由于叠氮化物对过氧化氢酶的抑制而导致的测量误差的方法,其中,源自被测成分以外的成分的过氧化氢被过氧化氢酶分解。用于减少由于叠氮化物对过氧化氢酶的抑制而导致的测量误差的方法,是使用过氧化氢,该过氧化氢酶的分子质量为75kDa以上,并且源自微生物,其中过氧化氢来源于被测定成分以外的成分。通过过氧化氢酶分解α-β-内酰胺,然后定量源自待测组分的过氧化氢以定量待测组分。

著录项

  • 公开/公告号US10415077B2

    专利类型

  • 公开/公告日2019-09-17

    原文格式PDF

  • 申请/专利权人 YUHKO HIRAO;

    申请/专利号US20080677676

  • 发明设计人 YUHKO HIRAO;

    申请日2008-09-11

  • 分类号C12Q1/30;

  • 国家 US

  • 入库时间 2022-08-21 12:17:02

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