Methods for normalizing background effects in multiplex immunoassays

摘要

Disclosed herein methods, compositions, and kits for obtaining a normalized measured amount of a target in a sample by measuring the amount of the target in the sample by contacting the sample with a detection reagent, measuring the amount of a ligand known to be absent in the sample being tested by contacting the sample with a control reagent, and obtaining the normalized measured amount of the target by calculating the ratio of the measured amount of the target to the measured amount of the ligand.