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Luciferin derivatives from bicyclic reactants and aminothiol derivatives and methods of use thereof
Luciferin derivatives from bicyclic reactants and aminothiol derivatives and methods of use thereof
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机译:来自双环反应物的荧光素衍生物和氨基硫醇衍生物及其使用方法
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摘要
The present disclosure features a condensation reaction and a luciferin-unmasking reaction that can be carried out under physiological conditions. In general, the condensation reaction involves reacting a bicyclic reactant with an aminothiol derivative, generating a luciferin or luciferin derivative. A luciferin can provide detectable luminescence. A luciferin derivative can be unmasked to provide detectable luminescence in a luciferin-unmasking reaction. The present disclosure provides bicyclic reactants and aminothiol derivatives suitable for use in the condensation reaction. The condensation and luciferin-unmasking reactions find use in a variety of applications, which are also provided.
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