首页> 外国专利> Method of manufacturing protein array or peptide array, method of identifying functional protein or functional peptide, protein array or peptide array, and functional protein or functional peptide identification kit

Method of manufacturing protein array or peptide array, method of identifying functional protein or functional peptide, protein array or peptide array, and functional protein or functional peptide identification kit

机译:蛋白质阵列或肽阵列的制造方法,功能蛋白质或功能肽的鉴定方法,蛋白质阵列或肽阵列,以及功能蛋白质或功能肽鉴定试剂盒

摘要

There is provided a method of manufacturing a protein array or peptide array suitable for an efficient screening of a functional protein or functional peptide. The method of manufacturing a protein array or peptide array includes the steps of: (a) preparing a nucleic acid immobilized on a solid support and a cell-free synthesis system in a reactor, in which a reactor array includes the reactor having a specific aperture shape and a protein capture molecule or a peptide capture molecule provided on at least a portion of wall surface and bottom surface in the reactor; and (c) synthesizing a protein or peptide from the nucleic acid using the cell-free synthesis system and immobilizing the protein or peptide in the reactor.
机译:提供了一种制造蛋白质阵列或肽阵列的方法,所述蛋白质阵列或肽阵列适于有效筛选功能性蛋白质或功能性肽。制造蛋白质阵列或肽阵列的方法包括以下步骤:(a)在反应器中制备固定在固相支持物上的核酸和无细胞合成系统,其中反应器阵列包括具有特定孔的反应器在反应器的壁表面和底表面的至少一部分上设置形状和蛋白质捕获分子或肽捕获分子; (c)使用无细胞合成系统从核酸合成蛋白质或肽,并将蛋白质或肽固定在反应器中。

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