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REAL-TIME REVERSE TRANSCRIPTASE-POLYMERASE CHAIN REACTION ASSAY WITH MODIFIED PROBE FOR THE DIAGNOSIS OF RABIES VIRUSES AND OTHER LYSSAVIRUSES
REAL-TIME REVERSE TRANSCRIPTASE-POLYMERASE CHAIN REACTION ASSAY WITH MODIFIED PROBE FOR THE DIAGNOSIS OF RABIES VIRUSES AND OTHER LYSSAVIRUSES
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机译:实时逆转录酶-聚合酶链反应分析与改良探针诊断狂犬病病毒和其他溶血病毒
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摘要
A real-time reverse transcriptase-polymerase chain reaction (RT-PCR) assay that utilizes multiplex primers and probes with degenerate nucleotides to detect divergent species of lyssavirus is described. The probes used in the RT-PCR assay target a highly conserved region at the 5′ end of the lyssavirus genome and are modified with either a minor groove binder (MGB) or locked nucleic acid (LNA) nucleotides to increase their melting temperature. The described assay detects all known lyssavirus species with a sensitivity and specificity superior to traditional hemi-nested PCR and the direct fluorescent antibody (DFA) test.
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