A kind of DNA probe sequence and acquisition methods for litopenaeus vannamei genetic sex identification

摘要

The invention discloses the DNA sequence dnas and acquisition methods of a kind of litopenaeus vannamei male and female gender differences. Pass through high-flux sequence means, high-flux sequence is carried out to the mixed pond of female individuals and male respectively, sequencing result filters out by bioinformatic analysis and mixes the sequence fragment that there were significant differences in pond in male and female, the differential fragment of acquisition is verified in the individual of separate sources, it is final to obtain the probe sequence that can be used for the examination of litopenaeus vannamei male and female, the precise Identification of this kind of prawn genetic sex can be realized using the sequence.