首页> 外国专利> DNA PROBE SEQUENCE FOR GENETIC SEX IDENTIFICATION OF LITOPENAEUS VANNAMEI AND ACQUISITION METHOD

DNA PROBE SEQUENCE FOR GENETIC SEX IDENTIFICATION OF LITOPENAEUS VANNAMEI AND ACQUISITION METHOD

机译：南美白对虾遗传性别鉴定的DNA探针序列及获得方法

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摘要

The present invention relates to a DNA sequence showing difference between the female and male individuals of Litopenaeus vannamei and its corresponding acquired method. The method comprises: respectively conducting high-through put sequencing on the mixing pools of female individuals and male individuals in by high-through put sequencing platform; conducting bioinformatic analysis on the sequencing results to screen out sequences showing significant difference between the mixing pools of female and male individuals; verifying the obtained sex difference sequence in individuals from different sources; and finally, obtaining a probe sequence for female and male identification of L. vannamei, so that the genetic sexe of this species can be accurately identified using the sequences. The method of the present invention has the characteristics of high efficiency, accuracy and reliability, and possess broad application potential in the early sex identification and sex control research of prawns.

机译：本发明涉及一种显示凡纳滨对虾（Itopenaeus vannamei）的雌性和雄性差异的DNA序列及其相应的获得方法。该方法包括：通过高通量测序平台分别在男女个体混合池中进行高通量测序。对测序结果进行生物信息学分析，以筛选出显示出男女混合池之间有显着差异的序列;验证来自不同来源的个体中获得的性别差异序列;最后，获得用于鉴定 L的雌性和雄性的探针序列。 vannamei ，以便可以使用序列准确识别该物种的遗传性别。本发明方法具有高效，准确，可靠的特点，在对虾的早期性别识别和性别控制研究中具有广阔的应用潜力。 展开▼

著录项

公开/公告号US2017175188A1

专利类型

公开/公告日2017-06-22

原文格式PDF

申请/专利权人 INSTITUTE OF OCEANOLOGY CHINESE ACADEMY OF SCIENCES;
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申请/专利号US201515322307

发明设计人 XIAOJUN ZHANG;FUHUA LI;YANG YU;JIANHAI XIANG;
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申请日2015-12-04

分类号C12Q1/68;

国家 US

入库时间 2022-08-21 13:50:52

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1. 一种用于凡纳滨对虾遗传性别鉴定的DNA探针序列及获取方法 [P] . 中国专利： CN104789690B . 2017.03.08

2. 一种用于凡纳滨对虾遗传性别鉴定的DNA探针序列及获取方法 [P] . 中国专利： CN104789690A . 2015-07-22

3. A kind of DNA probe sequence and acquisition methods for litopenaeus vannamei genetic sex identification [P] . 美国专利： US10280460B2 . 2019-05-07

机译：一种南美白对虾遗传性别鉴定的DNA探针序列及获取方法

4. DNA PROBE SEQUENCE FOR DETERMINING GENETIC SEXES OF LITOPENAEUS VANNAMEI AND OBTAINMENT METHOD [P] . 世界知识产权组织专利： WO2016180009A1 . 2016-11-17

机译：凡纳滨对虾遗传性的DNA探针序列测定及获得方法

5. A POLYPEPTIDE CODING MOLECULE OF THE NUCLEIC ACID, HUMAN GROWTH PROTEIN CODED BY THE DNA MOLECULE, HUMAN GROWTH PROTEIN CODING CDNA, PHARMACEUTICAL COMPOUND, APPLICATION OF HUMAN GROWTH PROTEIN AND THE DNA SEQUENCE, METHOD FOR THE DETERMINATION OF THE LOW GROWTH, METHOD FOR THE IDENTIFICATION OF THE GENETIC DEFECT, CELLS TRANSFORMED BY THE DNA SEQUENCE, TESTING SYSTEM AND METHOD FOR THE IDENTIFICATION OR SCREENING TESTS, EXPRESSION VECTOR AS WELL AS APPLICATION OF THE HUMAN GROWTH PROTEINS [P] . PL194248B1 . 2007-05-31

机译：核酸的多肽编码分子，由DNA分子编码的人类生长蛋白质，人类生长蛋白质编码CDNA，药物化合物，人类生长蛋白质和DNA序列的应用，低生长度的测定方法，方法基因缺陷，DNA序列转化的细胞，鉴定或筛选试验的表达系统和方法，表达载体以及人类生长蛋白的应用

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10. Development of a Blocking Primer to Inhibit the PCR Amplification of the 18S rDNA Sequences of Litopenaeus vannamei and Its Efficacy in Crassostrea hongkongensis [O] . Cong Liu, Ren-Jie Qi, Jing-Zhe Jiang, 2019

11. 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