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Recombinant Bacillus Subtilis for Increasing Production of Acetylglucosamine and Construction Method Thereof
Recombinant Bacillus Subtilis for Increasing Production of Acetylglucosamine and Construction Method Thereof
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机译:用于提高乙酰氨基葡萄糖产量的重组枯草芽孢杆菌及其构建方法
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摘要
The invention discloses a method for improving the yield of Bacillus subtilis acetylglucosamine, which belongs to the technical field of genetic engineering. In the invention, the recombinant Bacillus subtilis S5 (S5-PxylA-glmS-P43-GNA1) is taken as a starting strain, and the glmS ribozyme is integrated into the mid of rbs and the promoter sequence of the glmM and pfkA gene, respectively. The ribozyme mutant has the advantage of prolonging the stability of the mRNA and integrated into the mid of rbs and the promoter sequence of the pgi gene. The yield of GlcNAc of the recombinant strain reaches 11.79-20.05 g/L. This laid the foundation for the further metabolic engineering of Bacillus subtilis to produce GlcNAc.
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机译:本发明公开了一种提高枯草芽孢杆菌乙酰葡萄糖胺产量的方法,属于基因工程技术领域。在本发明中,将重组枯草芽孢杆菌S5(S5-PxylA-glmS-P43-GNA1)作为起始菌株,并将glmS核酶整合入rbs的中间,并启动子序列的构建。分别是glmM和pfkA基因。核酶突变体具有延长mRNA稳定性并整合到rbs和pgi基因启动子序列中间的优势。重组菌株GlcNAc的产量达到11.79-20.05 g / L。这为枯草芽孢杆菌 I>的进一步代谢工程生产GlcNAc奠定了基础。
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