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METHODS AND COMPOSITIONS FOR MAINTENANCE OF HEMATOPOIETIC STEM CELLS WITH PRESERVATION OF SELF-RENEWAL CAPACITY
METHODS AND COMPOSITIONS FOR MAINTENANCE OF HEMATOPOIETIC STEM CELLS WITH PRESERVATION OF SELF-RENEWAL CAPACITY
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机译:维持自我更新能力的造血干细胞的维持方法和组成
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摘要
Disclosed herein are methods of culturing hematopoietic stem cells (HSCs) that enhance preservation of the HSCs self-renewal capacity and multipotency for extended periods of time. Specific embodiments comprise culturing methods that involve use of a unique low calcium media. Certain embodiments comprise methods that enable the maintenance of multipotent and self-renewing HSCs for over two weeks. Certain embodiments avoid the use of chemicals that have the risk of unexpected off-target effects or mutagenicity.
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