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Expression and Purification of CRM Proteins and Related Proteins, and Protein Domains
Expression and Purification of CRM Proteins and Related Proteins, and Protein Domains
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机译:CRM蛋白及相关蛋白和蛋白结构域的表达和纯化
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摘要
The present invention is directed to the cells, compositions and methods for the production of recombinant protein. In particular, the invention is directed to a production process for obtaining high levels of soluble recombinant CRM197 protein from E. coli. Cells preferably contain one or more mutations of disulfide reductase genes, so that disulfide reductase activity is reduced. The invention also relates to purification method for CRM197 as well as characterization of properly folded CRM197 protein.
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