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Expression and purification of CRM proteins and related proteins, and protein domains

机译：CRM蛋白及相关蛋白和蛋白结构域的表达和纯化

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摘要

The present invention is directed to the cells, compositions and methods for the production of recombinant protein. In particular, the invention is directed to a production process for obtaining high levels of soluble recombinant CRM₁₉₇protein from E. coli. Cells preferably contain one or more mutations of disulfide reductase genes, so that disulfide reductase activity is reduced. The invention also relates to purification method for CRM₁₉₇as well as characterization of properly folded CRM₁₉₇protein.

机译：本发明涉及用于生产重组蛋白的细胞，组合物和方法。特别地，本发明涉及用于从 E获得高水平的可溶性重组CRM _{197 蛋白的生产方法。大肠杆菌。细胞优选包含二硫键还原酶基因的一个或多个突变，从而二硫键还原酶活性降低。本发明还涉及CRM _{197 的纯化方法以及正确折叠的CRM _{197 蛋白的表征。}}} 展开▼

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公开/公告号US10597664B2

专利类型

公开/公告日2020-03-24

原文格式PDF

申请/专利权人 FINA BIOSOLUTIONS LLC;
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申请/专利号US201816154020

发明设计人 NATALIA OGANESYAN;ANDREW LEES;
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申请日2018-10-08

分类号A61K39;A61K39/385;A61K39/02;A61K39/40;A61K48;A01N63;C12N15/70;C07K14/34;C07K1/22;C07K14/33;C07K14/235;A61K47/60;C12N9/02;C12N9/06;A61K38;

国家 US

入库时间 2022-08-21 11:29:56

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