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BASE SEQUENCE DETERMINATION APPARATUS, CAPILLARY ARRAY ELECTROPHORESIS APPARATUS, AND METHOD

机译:碱基序列测定装置,毛细管阵列电泳装置及方法

摘要

A base sequence determination apparatus includes (1) a mobility correction unit that outputs a mobility correction signal obtained by mobility correction of a time-series signal of a wavelength spectrum corresponding to each base, (2) a deconvolution unit that executes processes for calculating a deconvoluted signal of the mobility correction signal respectively for a plurality of parameter candidates of point spread function, calculating variance of peak intervals for the calculated deconvoluted signal, specifying a parameter of the point spread function using the calculated variance, and outputting the deconvoluted signal corresponding to the point spread function having the specified parameter as an updated deconvoluted signal, (3) a peak extracting unit that extracts a peak waveform from the updated deconvoluted signal and outputs an updated peak-extracted signal, and (4) a sequence specifying unit that inputs the updated peak-extracted signal and determines a base sequence.
机译:基本序列确定设备包括:(1)迁移率校正单元,其输出通过对与每个碱基相对应的波长谱的时序信号的迁移率校正而获得的迁移率校正信号;(2)执行用于计算α针对点扩展函数的多个参数候选分别进行迁移率校正信号的去卷积信号,计算所计算出的去卷积信号的峰间隔的方差,使用所计算出的方差指定点扩展函数的参数,并输出与具有指定参数的点扩展函数作为更新后的去卷积信号;(3)峰值提取单元,其从更新后的去卷积信号中提取峰值波形,并输出更新后的峰提取信号;以及(4)序列指定单元,其输入更新后的峰提取信号并确定碱基序列。

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