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compositions and methods for modifying gene expression using water deficit-inducible soybean promoter in plants

机译:水分亏缺型大豆启动子的植物组合物和修饰基因表达的方法

摘要

compositions and methods for modifying gene expression using water deficit-inducible soybean promoter in plants. The present invention relates to a nucleotide sequence capable of modifying the expression of one or more genes of interest in plant leaves and roots, primarily soybeans, as well as for producing genetically modified individuals that are capable of drought tolerance. More specifically, this invention relates to a novel soybean promoter which can be used to control expression of the coding sequence of genes of interest that confer tolerance to water deficit only at the time that transformed plants are subjected to stress conditions. , avoiding the constitutive expression of the transgene and unnecessary energy expenditure. In this context, this technology can be applied to biotechnological development and production of cultivars, especially soybean cultivars, which are more drought tolerant, without prejudice to plant development and yield. Thus, the present invention describes DNA constructs containing the promoter of the invention for the development of genetically modified plants, as well as a method for modifying gene expression in plants.
机译:水分亏缺诱导型大豆启动子在植物中修饰基因表达的组合物和方法。本发明涉及一种核苷酸序列,该核苷酸序列能够修饰植物叶和根(主要是大豆)中的一个或多个目的基因的表达,并且能够产生能够耐受干旱的基因修饰的个体。更具体地说,本发明涉及一种新颖的大豆启动子,该启动子可用于控制目的基因的编码序列的表达,该目的基因仅在使转化植物经受胁迫条件时才赋予对水分缺乏的耐受性。 ,避免了转基因的组成型表达和不必要的能量消耗。在这种情况下,该技术可用于生物技术开发和生产的品种,特别是大豆品种,其更耐旱,而不会损害植物的发育和产量。因此,本发明描述了含有用于遗传修饰植物的发育的本发明启动子的DNA构建体,以及修饰植物中基因表达的方法。

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