variant igg4 antibody, cell line, methods for modifying an igg antibody to reduce binding to a process impurity and for producing an igg4 antibody with reduced binding to the host cell protein, and, composition.

摘要

The present invention relates to variant antibodies and methods for generating said antibodies with a low level of binding to process impurities. in particular, the invention describes variant igg4 antibodies that have been modified in the heavy chain constant region at either amino acid or a combination of amino acids in the region between kabat 203 and 256 residues, with variant igg4 antibody having a reduced level. binding to host cell protein (hcp) as compared to an unmodified igg4 antibody. The invention also relates to compositions comprising said variant IgG4 antibodies.