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SELECTIVE AMPLIFICATION OF DESIRED NUCLEIC ACID AREAS IN A TARGET SEQUENCE
SELECTIVE AMPLIFICATION OF DESIRED NUCLEIC ACID AREAS IN A TARGET SEQUENCE
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机译:目标序列中期望的核酸区域的选择性扩增
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摘要
A method and kit for selective amplification of desired amplicons in a two-stage polymerase chain reaction is provided. The method and kit uses target primers comprising mismatch control sequences; wherein any pair of primers that flank a desired amplicon sequence have non-matching mismatch control sequences. and any pair of primers that flank an undesired amplicon sequence have matching mismatch control sequences.
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