Helper plasmid, the strain and the system of the broad host spectrum plasmid mobilization and use thereof

摘要

The invention relates to a helper plasmid for mobilization, which comprises a mobilizing part of the pCTX-M3 plasmid conjugation system (tra and trb region genes) and a bacterial strain for efficient broad host range mobilization of plasmids that comprises a mobilizing part of the conjugation system (tra and trb region genes) integrated into the chromosome. The invention also relates to efficient, plasmid broad host range mobilization systems that comprise the plasmid or the bacterial strain and the mobilized plasmid that contains an oriT sequence compatible with the system.