Spectrum of Glycidyl Methacrylate-induced Mutation in Plasmid-Escherichia coli System

摘要

In order to chitracterizc the speclrum of mutallon indueed by glyeidyl methacrylule(GMA ), the plasmid pBR322 was modificed with this mutagen in vitro , transttclcd intoapproprate Escherichia colii host HB101. The mutants were then sereened and defined by DNA sequencing . Sequence analysis reveals that G MA induces two classes of mulations deletion of the mono-. di’ or fetra-base or the insertion of mono’ or di-base. Both typesof mutations, with about 10 % frequency. occur predominantly at C-G runs and at5’-CNCCN-3’ sequence ,which are hotspots for GMA damage and may cause frameshift mutation.