Method for pretreatment of silage, preferably maize-silage and consortium of microorganisms for pretreatment of silage, preferably maize-silage

摘要

Method of preparation of the silage, in particular maize silage, in which the silage is removed from the container and fed to the macerator,where it is mixed with liquid material and mechanically crushed, the resulting mixture is transferred to a biotechnological accelerator, preferably in ports,where the temperature is maintained at the assumed level and the acidic environment is maintained in the accelerator by promoting the occurrence of biochemical changes in the fermentation process,in particular hydrolysis and acidogenesis processes, and preferably also octanogenesis,where the acidification process in the accelerator is obtained by overloading the accelerator chamber with the dry organic mass of the substrate and maintaining the dry mass content of the insert at the assumed level,in addition, carbon dioxide and hydrogen released into the accelerator during the biochemical transformation of the hydrolysis process shall be fed to the main fermentation tank,Whereas biochemical processes carried out by a specific consortium of micro -organisms are enhanced by a beneficial mixture which is less intense and symmetrically balanced in its total volume,the accelerator tank is characterised by a temperature in the biotechnological accelerator, which is maintained within 20 -65oC, favourable 35 -42oC,in addition, the acid environment in the accelerator is maintained at pH 3 -5, favourable in pH 3,5 -4,6,the dry organic mass of the substrate is controlled within 30 -150 kg -m3 -d -1, keeping the dry mass content in the inlet at 12 -20%,favourable in Section 14 -16%,Whereas the selection of the values of the individual parameters of the pre -treatment method of silage is considered optimal when a specific consortium of micro -organisms is maintained in the accelerator pulp.The notification also concerns a consortium of micro -organisms whose structure at the type level is expressed as the following percentage of individual micro -organisms: Firmicutes 50 -65%,Actinobacteria 5 - 25%, Proteobacteria 2 - 20%, Bacterioidetes 30%,where the consortium of micro -organisms has a class structure expressed by the following percentage of individual micro -organisms: Clostridia 30%, Bacteroidia 30%, Bacilli 5 -50%,Alphaproteobacteria 2 - 10%, Gamma Proteobacteria 2 -8%, Betaprotobacteria 2 - 5%, Acinobacteria 5 -25%, Flavobacteria 1 - 3%, Cloacamonae 1%, Verrucomycrobiae 1%,where the specific consortium of micro -organisms has a structure at the level of the following percentage of individual micro -organisms: Lactobacillales 15 -45%,Bifidobacteriales 5 - 25%, Rhodspiriales 2 - 8%, Bacillales 1 - 5%, Actinomycetales 1 - 5%, Byrkholderiales 1 - 8%, Clostridiales 25%, Bacteriodales 30%, Cloacamonales 2%.