Method for identification of mutations in gene SLC16A1 as the marker for prediction of the racing capability in Arabian horses and the marker obtained by this method

摘要

The purpose of the notification is to identify the mutation in the SLC16A1 gene, the prediction marker for the racing potential of Arab horses is to:that the blood from the cervical vein of the horse is collected into the test tube with an EDTA anticoagulant and then from the biological material thus prepared the genomic DNA is isolated and suspended in the TE buffer,spectrophotometric estimation of the quality and quantity of nucleic acid,and then the DNA samples in the TE buffer are diluted to obtain the 100 ng /ul input matrix concentration for the modified HRM response,the total volume of the reaction vessel and the molar concentration of the starters in the final mixture are reduced and the PCR -HRM is corrected using properly prepared starters; ored a reaction mixture and a temperature -time profile. The notification shall also include a marker obtained in accordance with the above technical parameters and designated as a mutation g.55589063T G.