Biosurfactants of stratospheric bacillus: production, characterization and potential of Biotechnology

摘要

The invention relates to the preparation of a novel halophilic peptide produced by Bacillus sp. The strain was isolated from the oil polluted sea water of Sfax fishing port in Tunisia. After enrichment of fluorine, it was a polycyclic aromatic hydrocarbon (HAP) composed of three aromatic nuclei and one cyclopentane. In the absence of yeast extract and 30 g / L NaCl. F-5 strain can also grow on a variety of 'aliphatic hydrocarbons, aromatic hydrocarbons and complexes ("octane", "BTEX").Bile acid, gentian acid, Pyrenees, crude oil, fuel Page: 1As the only source of carbon and energy Biosurfactant is produced by surface tension measurement and oil displacement test in the presence of different carbon sources. Flo5 strain showed its ability to produce biosurfactant on most of the test substrates, especially on residual fried oil as a low-carbon source.Therefore, the high cost of producing these surfactants is minimized. The biosurfactant was purified by silica gel column chromatography and membrane chromatography. MALDI-TOF / MS analysis showed that the biosurfactants were lipopeptides, especially surfactants and PUMIC acid. Critical micelle concentrationFl5 purified fat peptide is about 50 mg / L, which can reduce the surface tension of water from 72 mg / s to 28 mg / s, and in this case, the surface tension is reduced to about 34 mg / s. "The study on the stability of crude oil bs-flu5 showed that the stability of bs-flu5 remained at 121 ° C after incubation.The stability of pH was not affected by pH 2.1 to pH 12. On salinityThe surface voltage remained stable until the high concentration of NaCl (120 g / L). Cytotoxicity studyThe results showed that bs-flu5 biosurfactant had no toxic effect on human HEK cellsBiosurfactants produced by fluorochloro-5 strain were applied to the dissolution of hydrocarbons in soil contaminated by waste engine oil using residual fried oil as carbon source. It was found that they were more effective than some surfactants. Tested chemicals: these hydrocarbon biodegradability and the potential production of fluoro-5 biosurfactants are high and can be used to develop strategies for bioremediation of hydrocarbon contaminated water and marine sediments,And other applications in various fields, such as food processing, cosmetics, agriculture, etc.