HIGH RESOLUTION SYSTEMS, KITS, APPARATUS, AND METHODS FOR SCREENING MICROORGANISMS AND OTHER HIGH THROUGHPUT MICROBIOLOGY APPLICATIONS

摘要

INTERNATIONAL APPLICATION PUBLISHED UNDER THE PATENT COOPERATION TREATY (PCT) (19) World Intellectual Property -,` Organization MD HIM 01E101011M 3E1 0 III11101111 100 HOE NH ill OEN International Bureau (10) International Publication Number 3 (43) International Publication Date .....••'' WO 2018/075701 Al 26 April 2018 (26.04.2018) WIP0 I PCT (51) International Patent Classification: Kimberly; 29 Fleetwood Drive, Daly City, California C12M 1/04 (2006.01) C12Q 1/00 (2006.01) 94015 (US). C12M 1/34 (2006.01) C12Q 1/68 (2018.01) (74) Agent: SUN-HOFFMAN, Lin; P.O. Box 52048, Palo Alto, (21) International Application Number: California 94303 (US). PCT/US2017/057279 (81) Designated States (unless otherwise indicated, for every (22) International Filing Date: kind of national protection available): AE, AG, AL, AM, 19 October 2017 (19.10.2017) AO, AT, AU, AZ, BA, BB, BG, BH, BN, BR, BW, BY, BZ, CA, CH, CL, CN, CO, CR, CU, CZ, DE, DJ, DK, DM, DO, (25) Filing Language: English DZ, EC, EE, EG, ES, FI, GB, GD, GE, GH, GM, GT, HN, (26) Publication Language: English HR, HU, ID, IL, IN, IR, IS, JO, JP, KE, KG, KH, KN, KP, (30) Priority Data: KR, KW, KZ, LA, LC, LK, LR, LS, LU, LY, MA, MD, ME, 62/410,337 19 October 2016 (19.10.2016) US MG, MK, MN, MW, MX, MY, MZ, NA, NG, NI, NO, NZ, OM, PA, PE, PG, PH, PL, PT, QA, RO, RS, RU, RW, SA, (63) Related by continuation (CON) or continuation-in-part SC, SD, SE, SG, SK, SL, SM, ST, SV, SY, TH, TJ, TM, TN, (CIP) to earlier application: TR, TT, TZ, UA, UG, US, UZ, VC, VN, ZA, ZM, ZW. US 15135377 (CIP) (84) Designated States (unless otherwise indicated, for every Filed on 21 April 2016 (21.04.2016) kind of regional protection available): ARIPO (BW, GH, — (71) Applicant: GENERAL AUTOMATION LAB TECH- GM, KE, LR, LS, MW, MZ, NA, RW, SD, SL, ST, SZ, TZ, NOLOGIES, INC. [US/US]; 953 Indiana Street, Suite 372, UG, ZM, ZW), Eurasian (AM, AZ, BY, KG, KZ, RU, TJ, San Francisco, California 94107 (US). TM), European (AL, AT, BE, BG, CH, CY, CZ, DE, DK, EE, ES, FI, FR, GB, GR, HR, HU, IE, IS, IT, LT, LU, LV, Inventors: HALLOCK, Alexander; 105 Camellia Av- (72) MC, MK, MT, NL, NO, PL, PT, RO, RS, SE, SI, SK, SM, enue, Redwood City, California 94061 (US). WEMMER, = (54) Title: HIGH RESOLUTION SYSTEMS, KITS, APPARATUS, AND METHODS FOR SCREENING MICROORGANISMS OTHER HIGH THROUGHPUT MICROBIOLOGY APPLICATIONS AND = = = = = ,-- .., -____ _ = /1117 • 0 illii _ • C:ZZz:22, 00,,,,, ologal oop l gittar • c o „,,, o 0474:7 _ --:: ,.' PoogPa ' ,';' ,Pz 112,2 7§7 27 •egrdiegeg ;.- 0 0 , 76 -811 gaboaggicti • • fapszt, ~ O poo 0 0 p0 ho sze o f = = = = _ = _ • -•, Il - ---- ,-• Il 0 N FIG. 1 11 in N © --- -- GO (57) : A microfabricated device defining a high density array of microwells is described for cultivating cells from a sample. ,-1 The device may be incubated to grow a plurality of cells, which may be split into an analysis portion and a reserve portion. Methods are 0 provided for screening a biological entity of interest using the microfabricated device, for example, for screening phosphate solubilizing " bacteria or other bacteria producing acids. [Continued on next page] WO 2018/075701 Al MIDEDIMOM0101011EFIDINIIHIEDOEDIROIIMOVOIMIE TR), OAPI (BF, BJ, CF, CG, CI, CM, GA, GN, GQ, GW, KM, ML, MR, NE, SN, TD, TG). Published: — with international search report (Art. 21(3)) — with sequence listing part of description (Rule 5.2(a))