Physical self-organizing hydrogel system for biotechnological applications

摘要

Physical self-organizing hydrogel system for biotechnological applications consisting of a non-covalent network based on a protein-ligand interaction, comprising a tetrameric protein as a protein and biotin or one of its derivatives as a ligand, biotin or one of its derivatives being covalently conjugated in each case to one end of a polymeric chain of a single or double-stranded oligonucleotide, and the total content of solids related to the hydrogel totaling at least 3% by mass and the conjugates being crosslinked by the tetrameric protein, where in In the hydrogel system, there is a mixing ratio, as a molar ratio of equivalents between the protein and the number of terminal biotinylated ends of the polymer chains in the biotin-polymer conjugate, from 1: 2 to 1: 8.