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Method for measuring a spectral sample response

机译:测量光谱样本响应的方法

摘要

Method of measuring a spectral response of a sample (1), comprising the following steps: - generating a probe light that has a primary spectrum and that comprises probe light pulses (2) that are generated with a device laser source of fs (10) and having an impulse duration equal to or less than 100 fs, in which the primary spectrum covers a range of wavelengths including wavelengths of at least 3 μm, - irradiate the sample (1) with the probe light, which includes an interaction of the probe light and the sample (1), and - detecting by spectral resolution the probe light having a modified spectrum, which deviates from the primary spectrum as resulting from the interaction of the probe light and the sample (1), said modified spectrum being characteristic of the spectral response of the sample (1), in which - the detection step comprises sampling in the temporal domain a temporary form of the impulses s of probe light (2) after the interaction with the sample (1) and the spectral response of the sample (1) is obtained on the basis of a Fourier transformation of the temporal form of the probe light pulses ( 2) after the interaction with the sample (1), characterized in that - the sample comprises a biological sample (1), - the probe light pulses (2) are created with an average power above 50 mW, and after the interaction with the biological sample (1), each of the probe light pulses (2) comprises a main pulse corresponding to the primary spectrum of the probe light pulses and a temporal wake which is induced by absorption of the probe. the biological sample (1) and following the main pulse, in which sampling in the time domain of the temporal form of the probe light pulses (2) includes sampling the temporal wake.
机译:测量样品(1)的光谱响应的方法,包括以下步骤:-生成具有初级光谱并且包含由fs的设备激光源(10)生成的探测光脉冲(2)的探测光。并具有等于或小于100 fs的脉冲持续时间,其中主光谱覆盖包括至少3μm波长在内的一系列波长,-用探测光照射样品(1),包括探测光的相互作用光和样品(1),以及-通过光谱分辨率检测具有修改后的光谱的探测光,该探测光偏离了由于探测光和样品(1)的相互作用而产生的主光谱,所述修改后的光谱具有以下特征:样品(1)的光谱响应,其中-检测步骤包括在与样品(1)相互作用和光谱响应之后在时域中对探测光(2)的脉冲形式的临时形式进行采样(1)的样品是在与样品(1)相互作用后基于探针光脉冲(2)的时间形式的傅立叶变换获得的,其特征在于-样品包括生物样品(1) --以平均功率高于50 mW产生探测光脉冲(2),并且在与生物样品(1)相互作用后,每个探测光脉冲(2)都包含一个与探针的光脉冲和由探针吸收引起的暂时性唤醒。生物样品(1)并跟随主脉冲,其中在探测光脉冲(2)的时间形式的时域中进行采样包括对时间唤醒进行采样。

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