Method for measuring a spectral sample response

摘要

Method of measuring a spectral response of a sample (1), comprising the following steps: - generating a probe light that has a primary spectrum and that comprises probe light pulses (2) that are generated with a device laser source of fs (10) and having an impulse duration equal to or less than 100 fs, in which the primary spectrum covers a range of wavelengths including wavelengths of at least 3 μm, - irradiate the sample (1) with the probe light, which includes an interaction of the probe light and the sample (1), and - detecting by spectral resolution the probe light having a modified spectrum, which deviates from the primary spectrum as resulting from the interaction of the probe light and the sample (1), said modified spectrum being characteristic of the spectral response of the sample (1), in which - the detection step comprises sampling in the temporal domain a temporary form of the impulses s of probe light (2) after the interaction with the sample (1) and the spectral response of the sample (1) is obtained on the basis of a Fourier transformation of the temporal form of the probe light pulses ( 2) after the interaction with the sample (1), characterized in that - the sample comprises a biological sample (1), - the probe light pulses (2) are created with an average power above 50 mW, and after the interaction with the biological sample (1), each of the probe light pulses (2) comprises a main pulse corresponding to the primary spectrum of the probe light pulses and a temporal wake which is induced by absorption of the probe. the biological sample (1) and following the main pulse, in which sampling in the time domain of the temporal form of the probe light pulses (2) includes sampling the temporal wake.