MASS SPECTROMETRY HISTOCHEMISTRY OF PEPTIDES FROM FORMALDEHYDE-FIXED, PARAFFIN-EMBEDDED TISSUE

摘要

The present invention in general relates to a method of localization andstructural characterization of peptides in aformaldehyde-fixed paraffin-embedded biological sample using matrix assistedionization (such as in MALDI). Specifically, the inventionrelates to the combination of the development of a sample preparation protocolwhich does not require any enzymatic digestionnor antigen-retrieval steps, with highly sensitive mass spectrometry.