MASS SPECTROMETRY HISTOCHEMISTRY OF PEPTIDES FROM FORMALDEHYDE-FIXED, PARAFFIN-EMBEDDED TISSUE

摘要

The present invention in general relates to a method of localization and structural characterization of peptides in a formaldehyde-fixed paraffin-embedded biological sample using matrix assisted ionization (such as in MALDI). Specifically, the invention relates to the combination of the development of a sample preparation protocol which does not require any enzymatic digestion nor antigen-retrieval steps, with highly sensitive mass spectrometry.