MULTIPLE COPY GENE CONSTRUCTS AND METHODS FOR THE RAPID GENERATION OF RECOMBINANT PROTEIN PRODUCTION CELL LINES

摘要

The present invention is directed to multiple-copy gene constructs comprising multiple gene constructs for use in recombinant protein-producing cells, wherein each gene copy in the multi- ple gene constructs encodes the identical protein of interest but varies from the other genes in the multiple copy gene construct by at least 0.5%, preferably at least 5 % in its DNA sequence. The multiple-copy gene constructs may comprise more than one set of multiple gene constructs, wherein each set of gene constructs encodes the identical protein of interest but different sets of gene constructs encode different proteins, e.g. multi unit proteins such as light and heavy chains of an antibody. In further aspects the invention also relates to corresponding vectors, cells, and methods for using the multiple-copy gene construct as well as a kit of parts for use in such methods.