RECOMBINANT METARHIZIUM ACRIDUM, PREPARATION METHOD THEREFOR AND USE THEREOF

摘要

Disclosed is a recombinant Metarhizium acridum capable of expressing phosphoglycerate kinase. The gene introduced in the recombinant Metarhizium acridum is the phosphoglycerate kinase gene, which can significantly increase the concentration of fructose-6-phosphate in the obligate bacterium, Metarhizium acridum, and significantly improve the insecticidal efficiency such that the median lethal time, LT50, of Metarhizium acridum is reduced from 7.045 ± 0.211 days to 5.617 ± 0.187 days. In addition, the recombinant Metarhizium acridum is harmless to the environment, biologically safe and non-toxic to humans.