METHOD FOR SCREENING GLUTAMINE SYNTHETASE-DEFICIENT HEK293 CELL LINE

摘要

Provided is an HEK293 cell-based glutamine synthetase-deficient cell line HEK293-GS-/- that can be stably passaged, adapted to a suspension culture, and is applicable to recombinant protein expression constructed using a CRISPR/Cas9 system. The cell line can express various recombinant proteins through screening using a GS/MSX screening system, can stably express a target protein after multiple passages, and can adapt to most commercial serum-free media.