METHOD FOR EVALUATING TRANSPORT OF NEGATIVELY CHARGED DRUGS ON EXPERIMENTAL MODEL OF HEK293 CELL LINE

摘要

FIELD: biotechnology; pharmacology; molecular biology.;SUBSTANCE: it can be used in the examination of drugs having a renal excretion pathway, as well as at the preclinical stage in the development of new drugs, the invention makes it possible to evaluate the transport of negatively charged drugs - substrates of OAT1 transporter by comparing expression levels of SLC22A6 gene of HEK293-OAT1 cell line by reverse transcription polymerase chain reaction.;EFFECT: obtaining a method for evaluating transport of negatively charged drugs on experimental model of HEK293 cell line.;1 cl, 9 dwg, 1 tbl, 4 ex