NEURAL CREST CELLS TO REVITALIZE CRANIAL ALLOGRAFTS

摘要

Described herein are methods and compositions related to use of cranium-specific induced pluripotent stem cell (iPSC)-derived cells that are demonstrated as capable of revitalizing structural allografts. Specifically, iPSC-derived neural crest cells (iNCCs) can be differentiated into mesenchymal stem cells (iNCC-MSCs) that cells are seeded on allografts. Coating cranial allografts with mesenchymal stem cells derived from induced neural crest cells, provides a new effective therapeutic avenue for cranial defects. This approach improves allograft function by combination with a reproducible and inexhaustible source of cranium-specific MSCs. The results shown herein demonstrate improved integration and revitalization of induced neural crest (iNCC-MSC)-coated allografts compared to bone marrow (BM)-MSE-coated allografts, both applied in combination with intermittent parathyroid hormone (PTH) therapy in a calavarial defect model.