SANDWICH ELISA FOR IDENTIFYING SUBJECTS WHO MAY BENEFIT FROM TREATMENT WITH THERAPEUTIC AGENTS

摘要

The present application relates to compositions and methods for the identification of subjects who are likely to benefit from use of a therapeutic agent. The present invention utilizes the specificity of therapeutic monoclonal antibodies for a single epitope to determine whether a therapeutic monoclonal antibody interacts with biological material in/from a patient. The targeting complex comprises 1. antibody A, wherein antibody A is a potential therapeutic monoclonal antibody, or fragment thereof, comprising (a) a variable region specific to an epitope of interest, and (b) a human constant region; and 2. antibody B, wherein antibody B recognizes the human constant region of antibody A.