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L-GLUTAMATE DEHYDROGENASE MUTANT AND APPLICATION THEREOF
L-GLUTAMATE DEHYDROGENASE MUTANT AND APPLICATION THEREOF
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机译:L-谷氨酸脱氢酶突变体及其应用
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摘要
Provided are an L-glutamate dehydrogenase mutant and an application thereof, the mutant mutating the amino acid residue A at position 166 and/or the amino acid residue V at position 376 shown in SEQ ID NO. 1 into a hydrophilic or small sterically hindered amino acid residue, the application performing an amination reaction of 2-oxo-4-(hydroxymethylphosphinyl)butyrate in the presence of an L-amino acid dehydrogenase mutant, an inorganic amino donor, and a reduced coenzyme NADPH, and performing an acidification reaction on the obtained L-glufosinate-ammonium salt to obtain L-glufosinate-ammonium. Compared to wild L-glutamate dehydrogenase, the present L-glutamate dehydrogenase mutant has a higher concentration of substrates that can be catalysed when preparing L-glufosinate-ammonium, thereby increasing the efficiency of the action of the enzyme and reducing reaction costs.
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机译:提供了L-谷氨酸脱氢酶突变体及其应用,该突变体突变了SEQ ID NO.6所示的166位的氨基酸残基A和/或376位的氨基酸残基V。在存在L-氨基酸脱氢酶突变体,无机氨基供体和还原型辅酶的情况下,本申请进行了2-羟基-(羟甲基膦基)丁酸酯的胺化反应NADPH,对得到的L-草铵膦铵盐进行酸化反应,得到L-草铵膦铵盐。与野生的L-谷氨酸脱氢酶相比,本发明的L-谷氨酸脱氢酶突变体具有更高浓度的底物,其在制备L-草铵膦-铵时可以被催化,从而提高了酶的作用效率并降低了反应成本。
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