METHODS FOR CANCER DETECTION BY EVALUATION OF GLYCAN-BINDING PATTERNS OF IMMUNOGLOBULINS IN GASTROINTESTINAL LAVAGE FLUID SAMPLES

摘要

Described herein are non- invasive or minimally invasive methods and compositions for collecting and assessing samples for detection of gastrointestinal (GI) tract cancers and pancreatic cancers. Samples comprising gastrointestinal lavage fluid (GLF) are obtained from subjects and screened against a glycan microarray to reveal disease- specific glycan- binding patterns by immunoglobulins contained in the GLF, especially immunoglobulin A (IgA). The disease-specific glycan-binding patterns include a disease- specific glycan or subset of glycans from the microarray that are bound by the immunoglobulins in GLF at highest intensities or at lowest intensities, as well as one or more glycan motifs found within this disease- specific subset of glycans.