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SEQUENTIAL SAMPLING METHOD FOR IMPROVING IMMUNOASSAY SENSITIVITY AND KINETICS OF SMALL VOLUME SAMPLES
SEQUENTIAL SAMPLING METHOD FOR IMPROVING IMMUNOASSAY SENSITIVITY AND KINETICS OF SMALL VOLUME SAMPLES
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机译:改善小体积样品免疫分析灵敏度和动力学的顺序采样方法
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摘要
The disclosure provides a method for an enhanced detection of an analyte present in a biological sample. After the formation of the analyte/specific binding member(s)/detectable label complex, the labels are eluted and a first aliquot of eluant is brought into contact with a solid support, wherein the solid support comprises immobilized thereto specific binding member that specifically binds to the label, removing the first aliquot from the solid support and contacting the solid support with a second aliquot of the eluted label, and repeating the above steps, such that the label is concentrated on the solid support for further analysis to quantify the analyte in the biological sample.
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