Co-culture Cell-derived Nanovesicle for Advanced Inducement of Cell-Cell Interaction and Use thereof

摘要

The present invention relates to a nanovesicle derived from cells for cocultivation to induce improved cell interaction, and uses thereof and, more specifically, to a nanovesicle derived from cells for cocultivation, a target cell regulation method using the same, and a tissue regeneration method, which are capable of replacing cells for cocultivation by inducing signal transmission through a contact between cells with improved efficacy. According to the present invention, the nanovesicle derived from cells for cocultivation enables quantification through various methods and does not require additional separation process like in existing cocultivation for cell interaction by being naturally removed as time goes since life activities (cell division) are not shown. Moreover, the present invention can be used to induce interaction between cells without a contamination between cells, which has been pointed out as a problem in a case of existing cocultivation. The nanovesicle of the present invention is useful in applied on various application fields by inducing interaction between cells without adhesion culture through diffusing characteristics unlike an existing cocultivation method through adhesion culture of secondary cells, which had difficulties in being used in applications such as bioreactor or blood cell culture requiring a suspension culture. According to the present invention, a composition for cell replacement to perform coculture includes the nanovesicle derived from cells for cocultivation.