iPSC Cell culture platform for single cell sorting and enhanced reprogramming of iPSCs

摘要

The present invention provides cell culture conditions for culturing stem cells, including nutrient-free conditions for producing and culturing human induced pluripotent stem cells (iPSCs). Specifically, the present invention relates to a method for the long-term culture of pluripotent cells, reprogramming cells in a nutrient-free environment, single cell separation of pluripotent cells, cell classification of pluripotent cells, maintenance of undifferentiated state, Thereby providing a culture platform that allows the production of natural universal cells.