iPSC Cell culture platform for single cell sorting and enhanced reprogramming of iPSCs

摘要

The present invention provides cell culture conditions for culturing stem cells, including a condition for producing and culturing human induced pluripotent stem cells (iPSCs) without feeder cells. Specifically, the present invention provides long-term cultivation of pluripotent cells, reprogramming of cells in an environment containing no feeder cells, single-cell separation of pluripotent cells, cell sorting of pluripotent cells, maintenance of non-differentiated state, improved efficiency of reprogramming, and It provides a culture platform that allows the production of natural pluripotent cells.